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Mirus Bio
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Kreatech Diagnostics
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Medical Supply Company LTD
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Kreatech Diagnostics
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Becton Dickinson
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Cambridge Bioscience
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BioMicro Systems Inc
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Bionova Inc
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Image Search Results
Journal: Molecular Pharmaceutics
Article Title: Optimizing the Delivery of mRNA to Mesenchymal Stem Cells for Tissue Engineering Applications
doi: 10.1021/acs.molpharmaceut.3c00898
Figure Lengend Snippet: Distribution of mRNA nanoparticles in a collagen-nHA scaffold. (A) Representative confocal scanning micrograph of Cy3-labeled mRNA nanoparticles (jetPEI-modRNA 5 μg 2:1 v/w) in a cross section of a collagen-nHA scaffold. mRNA nanoparticles are fluorescently tagged (red), and the collagen-nHA scaffold structure autofluoresces (blue). mRNA nanoparticles remained close to both scaffold surfaces, where they had initially been soak-loaded. Scale bar = 500 μm. (B) Higher-magnification confocal scanning micrograph of (i) Cy3-labeled mRNA nanoparticles within the nanoparticle-rich area of the scaffold demonstrating even distribution of nanoparticles throughout the scaffold pores. (ii) A blank collagen-nHA scaffold was imaged as a control. Scale bar = 100 μm.
Article Snippet: To assess the distribution of mRNA nanoparticles within a collagen-nHA scaffold, modRNA was tagged with Cy3 using a Mirus
Techniques: Labeling
Journal: International Journal of Nanomedicine
Article Title: Intravenous Delivery of Angiopep-Functionalized Polypropylenimine Dendriplex Enhances Gene Expression in the Brain
doi: 10.2147/IJN.S510487
Figure Lengend Snippet: Confocal microscopy imaging of the cellular uptake of fluorescein-labelled DNA (2.5 μg/well), either complexed to DAB-Ang dendrimer, DAB dendrimer, or in solution after 24 h incubation with Bend.3 cells. (Blue: nuclei stained with DAPI (excitation: 405 nm laser line, bandwidth: 415–491 nm), green: fluorescein-labelled DNA (excitation: 514 nm laser line, bandwidth: 550–620 nm), red: cell wall (excitation: 633 nm laser line (bandwidth: 650–685 nm)) (Bar: 30 μm).
Article Snippet: The
Techniques: Confocal Microscopy, Imaging, Incubation, Staining
Journal: International Journal of Nanomedicine
Article Title: Intravenous Delivery of Angiopep-Functionalized Polypropylenimine Dendriplex Enhances Gene Expression in the Brain
doi: 10.2147/IJN.S510487
Figure Lengend Snippet: Fluorescence emission intensity as a quantitative measurement of the cellular uptake of fluorescein-labelled DNA (2.5 μg/well) either complexed to DAB-Ang dendrimer, DAB dendrimer, or in solution after 24 h incubation with Bend.3 cells. Results are expressed as the mean ± SEM of n=3, *: P≤ 0.05 versus the highest level of gene expression.
Article Snippet: The
Techniques: Fluorescence, Incubation, Gene Expression
Journal: International Journal of Nanomedicine
Article Title: Intravenous Delivery of Angiopep-Functionalized Polypropylenimine Dendriplex Enhances Gene Expression in the Brain
doi: 10.2147/IJN.S510487
Figure Lengend Snippet: Confocal microscopy imaging for the assessment of the mechanism of cellular uptake of fluorescein-labelled DNA (2.5 μg/mL) complexed with DAB-Ang in Bend.3 cells after pre-treatment with various cellular uptake inhibitors. (Blue: nuclei stained with DAPI, green: fluorescein-labelled DNA, red: cell wall stained with Alexa Fluor 647 probe) (Bar: 30 μm).
Article Snippet: The
Techniques: Confocal Microscopy, Imaging, Staining
Journal: International Journal of Nanomedicine
Article Title: Intravenous Delivery of Angiopep-Functionalized Polypropylenimine Dendriplex Enhances Gene Expression in the Brain
doi: 10.2147/IJN.S510487
Figure Lengend Snippet: Fluorescence emission intensity as a quantitative measurement of the cellular uptake of fluorescein-labelled DNA (2.5 μg/well) complexed with DAB-Ang dendrimer in Bend.3 cells after pre-treatment with various cellular uptake inhibitors (n = 3), *: p < 0.05 compared with the positive control (no inhibitor).
Article Snippet: The
Techniques: Fluorescence, Positive Control